Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358384
Title Hyperthermophilic enzymes - stability, activity and implementation strategies for high temperature applications
Author(s) Unsworth, L.D.; Oost, J. van der; Koutsopoulos, S.
Source FEBS Journal 274 (2007)16. - ISSN 1742-464X - p. 4044 - 4056.
Department(s) Microbiological Laboratory
Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) archaeon pyrococcus-furiosus - extremely thermophilic archaebacteria - thermostable dna-polymerase - alpha-glucosidase gene - sulfolobus-solfataricus - escherichia-coli - biochemical-characterization - thermococcus-litoralis - thermotoga-maritima - protein stabili
Abstract Current theories agree that there appears to be no unique feature responsible for the remarkable heat stability properties of hyperthermostable proteins. A concerted action of structural, dynamic and other physicochemical attributes are utilized to ensure the delicate balance between stability and functionality of proteins at high temperatures. We have thoroughly screened the literature for hyperthermostable enzymes with optimal temperatures exceeding 100 °C that can potentially be employed in multiple biotechnological and industrial applications and to substitute traditionally used, high-cost engineered mesophilic/thermophilic enzymes that operate at lower temperatures. Furthermore, we discuss general methods of enzyme immobilization and suggest specific strategies to improve thermal stability, activity and durability of hyperthermophilic enzymes.
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