Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358393
Title Physiological and phylogenetic characterization of a stable chlorate-reducing benzene-degrading microbial community
Author(s) Weelink, S.A.B.; Tan, N.C.G.; Broeke, H. ten; Doesburg, W.C.J. van; Langenhoff, A.A.M.; Gerritse, J.; Stams, A.J.M.
Source FEMS Microbiology Ecology 60 (2007)2. - ISSN 0168-6496 - p. 312 - 321.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) gradient gel-electrophoresis - dechloromonas strain rcb - molecular characterization - sp-nov. - reductive dechlorination - biodegradation - bacteria - nitrate - degradation - toluene
Abstract stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. The benzene degradation rate was 1.65 mM benzene per day, which is similar to reported aerobic benzene degradation rates but 20¿1650 times higher than reported for anaerobic benzene degradation. Denaturing gradient gel electrophoresis of part of the 16S rRNA gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. Five bacterial clones were further analyzed. Two clones corresponded to bacteria closely related to Alicycliphilus denitrificans K601. The three other clones corresponded to bacteria closely related to Zoogloea resiniphila PIV-3A2w, Mesorhizobium sp. WG and Stenotrophomonas acidaminiphila. DGGE analysis of cultures grown with different electron donors and acceptors indicated that the bacterium related to Alicycliphilus denitrificans K601 is able to degrade benzene coupled to chlorate reduction. The role of the other bacteria could not be conclusively determined. The bacterium related to Mesorhizobium sp. WG can be enriched with benzene and oxygen, but not with acetate and chlorate, while the bacterium related to Stenotrophomonas acidaminophila grows with acetate and chlorate, but not with benzene and oxygen. As oxygen is produced during chlorate reduction, an aerobic pathway of benzene degradation is most likely.
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