Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358484
Title Intestinal translocation of Streptococcus suis type 2 EF+ in pigs
Author(s) Swildens, B.; Stockhofe-Zurwieden, N.; Meulen, J. van der; Wisselink, H.J.; Nielen, M.; Niewold, T.A.
Source Veterinary Microbiology 103 (2004)1-2. - ISSN 0378-1135 - p. 29 - 33.
Department(s) ASG Infectieziekten
Livestock Research
ID - Dier en Omgeving
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) bacterial translocation - gastrointestinal-tract - extracellular factor - released protein - strains - virulence - identification - flora
Abstract Sepsis with subsequent multisystem organ failure after translocation of bacteria from the gut is a serious risk associated with stress situations. We showed that intestinal bacterial translocation could be one of the pathways for pathogenic Streptococcus suis infections in the pig. In 24 piglets weighing 10¿14 kg, free of the extracellular factor (EF+) producing phenotype of S. suis serotype 2, a silicon canula was placed in the proximal jejunum to enable intestinal inoculation and bypassing the upper alimentary tract. The pigs were individually housed. After stress induction in 18 pigs by means of a truck drive in individual cages for 1 h, pigs were inoculated through the intestinal canula either with S. suis type 2 EF+ or with growth medium only, and put back in their original housing. The six not transported pigs were also inoculated with the same strain. To prevent oral self-infection, faeces were collected in a bag that was glued around the anus. Clinical and behavioral symptoms were recorded for 72 h post inoculation, and then the animals were sacrificed for pathological and bacteriological examination. In three animals, the inoculation strain was re-isolated from mesenterial lymph nodes and typically affected organs. No S. suis type 2 EF+ was detected by specific polymerase chain reaction (PCR) in any of the tonsil-swabs and -homogenates. We concluded that infection of the organs had taken place after bacterial translocation out of the gut and that the intestinal tract can be a porte d¿entree for S. suis type 2 EF+.
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