Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358560
Title Molecular detection of Fusarium foetens in begonia
Author(s) Weerdt, M. de; Zijlstra, C.; Brouwershaven, I.R. van; Leeuwen, G.C.M. van; Gruyter, J. de; Kox, L.F.F.
Source Journal of Phytopathology 154 (2006)11-12. - ISSN 0931-1785 - p. 694 - 700.
Department(s) Biointeracties and Plant Health
Laboratory of Phytopathology
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) amplification - hiemalis - dna
Abstract Fusarium foetens is a new destructive pathogen on Begonia. This fungus is morphologically difficult to distinguish from other known Fusarium species. Moreover, the currently used assays for identification of this species require isolation and growth of the fungus which make the process time consuming. This study describes the development of a gel-based conventional polymerase chain reaction (PCR) test and a real-time PCR test for fast, practical, sensitive and specific detection of F. foetens. The gel-based PCR test presented amplifies a 275-bp fragment of the elongation factor (EF)-1¿ region. The real-time PCR test was based on the mitochondrial small subunit sequence of F. foetens. Both tests are suitable to detect F. foetens in cultures as well as in infected plant materials and offer potential for routine detection purposes.
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