Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358964
Title Positional assembly of temperature-responsive biopolymers
Author(s) Teeuwen, R.L.M.; Zuilhof, H.; Wolf, F.A. de; Hest, J.C.M. van
Source Polymer preprints 48 (2007)2. - ISSN 0032-3934 - p. 996 - 997.
Department(s) Laboratory for Organic Chemistry
AFSG Biobased Products
Publication type Non-refereed article in scientific journal
Publication year 2007
Abstract Incorporation of enzymic cascade reactions in a microchannel for org. synthesis can be achieved if spatially controlled immobilization of a series of enzymes is possible. This project attempts positional assembly of enzymes using the inverse phase transition temp. (Tt) of elastin-like proteins (ELPs). Increasing the temp. results in aggregation and assembly of ELPs, which can be very precisely controlled. Protein engineering is used as a method to produce ELPs and their fusion proteins. These fusion proteins can be assembled onto surfaces modified with the complementary ELP, when the surface is heated above Tt. Two elastin variants were constructed that each showed the expected inverse transition behavior. Fusions of these ELPs with fluorescent proteins generated proteins with inverse transition behavior that could be controlled using varying concns. of NaCl. We are currently investigating the possibilities to functionalize microchannels with ELPs to enable positional assembly of proteins.
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