Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360280
Title Kinetically controlled refolding of a heat denatured hyperthermostable protein
Author(s) Koutsopoulos, S.; Oost, J. van der; Norde, W.
Source FEBS Journal 274 (2007)22. - ISSN 1742-464X - p. 5915 - 5923.
DOI https://doi.org/10.1111/j.1742-4658.2007.06114.x
Department(s) Physical Chemistry and Colloid Science
Microbiological Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) archaeon pyrococcus-furiosus - crystal-structure - thermodynamic properties - thermal-denaturation - citrate synthase - stability - temperature - enzyme - water - dehydrogenase
Abstract The thermal denaturation of endo-ß-1,3-glucanase from the hyperthermophilic microorganism Pyrococcus furiosus was studied by calorimetry. The calorimetric profile revealed two transitions at 109 and 144¿°C, corresponding to protein denaturation and complete unfolding, respectively, as shown by circular dichroism and fluorescence spectroscopy data. Calorimetric studies also showed that the denatured state did not refold to the native state unless the cooling temperature rate was very slow. Furthermore, previously denatured protein samples gave well-resolved denaturation transition peaks and showed enzymatic activity after 3 and 9¿months of storage, indicating slow refolding to the native conformation over time.
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