Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360348
Title The early transcriptional response of pig small intestinal mucosa to invasion by Salmonella neterica serovar typhimurium DT104
Author(s) Niewold, T.A.; Veldhuizen, E.J.; Meulen, J. van der; Haagsma, H.P.; Wit, A.A.C. de; Smits, M.A.; Tersteeg, M.H.; Hulst, M.M.
Source Molecular Immunology 44 (2007)6. - ISSN 0161-5890 - p. 1316 - 1322.
Department(s) ID - Dier en Omgeving
Livestock Research
Animal Breeding and Genetics
LR - Backoffice
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) pancreatitis-associated protein - macrophage activation - epithelial-cells - gene-expression - up-regulation - in-vivo - infection - array - stimulation - sequence
Abstract Salmonella enterica serovar typhimurium (S. typhimurium) species are a leading cause of human invasive gastroenteritis. There is increasing in vitro evidence about Salmonella interaction with isolated cells or cell lines (macrophages, and enterocytes) on the molecular level, however, very little is known about in vivo interactions during actual invasion. We investigated the early interaction of S. typhimurium with intact small intestinal mucosa, in a pig model. Intestinal segments were infected with or without S. typhimurium DT104, and perfused. Whole mucosal gene expression was analyzed by cDNA array on 0, 2, 4, and 8 h post-infection. Invasion resulted in the upregulation of only eight transcripts in jejunal mucosa, among those the proinflammatory IL-8 (at 4 h only), and the antiinflammatory STAT3 (at 4 and 8 h). The limited number of differentially expressed genes found here in vivo compared to in vitro is most likely due to the presence of multiple, heterogenous cell interactions in intact mucosa. Furthermore, it is concluded that S. typhimurium evades strong host responses by downregulating the local inflammatory response
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