Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360349
Title Generation of EST and Microarray Resources for Functional Genomic Studies on Chicken Intestinal Health
Author(s) Hemert, S. van; Ebbelaar, B.H.; Smits, M.A.; Rebel, J.M.J.
Source Animal Biotechnology 14 (2003)2. - ISSN 1049-5398 - p. 133 - 143.
Department(s) Animal Breeding and Genetics
CIDC - Divisie Bacteriologie en TSE's
ID - Dier en Omgeving
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) expressed sequence tags - malabsorption-syndrome - disease resistance - gene-expression - mareks-disease - susceptibility - broilers - single - lines
Abstract Expressed sequenced tags (ESTs) and microarray resources have a great impact on the ability to study host response in mice and humans. Unfortunately, these resources are not yet available for domestic farm animals. The aim of this study was to provide genomic resources to study chicken intestinal health, in particular malabsorption syndrome (MAS), which affects mainly the intestine. Therefore a normalized and subtracted cDNA library containing more than 7000 clones was prepared. Randomly chosen clones were sequenced for control purposes. New ESTs were found and multiple ESTs not identified in the chicken intestine before were observed. The number of non-specific ESTs in this cDNA library was low. Based on this normalized and subtracted library a cDNA microarray was made. In a preliminary hybridization experiment with the microarray, genes were identified to be up- or downregulated in MAS infected chickens. This indicates that the generated resources are valuable tools to investigate chicken intestinal health by whole genome expression analysis approaches.
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