Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360620
Title In silico identification and mapping of microsatellite markers on Sus scrofa chromosome 4
Author(s) Wijk, H.J. van; Liefers, S.C.; Buschbell, H.; Dibbits, B.W.; Harlizius, B.; Groenen, M.A.M.
Source Animal Biotechnology 18 (2007)4. - ISSN 1049-5398 - p. 251 - 261.
Department(s) Animal Breeding and Genetics
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) carcass composition - porcine genome - linkage map
Abstract Apolipoprotein B (APOB) serves an essential role in the assembly and secretion of triglyceride-rich lipoproteins and lipids transport. This study was designed to clone the full-length cDNA of the chicken APOB gene, to characterize the expression profile, and investigate the differential expression between layer and broiler of the chicken APOB gene. The full-length cDNA sequence (14,150-bp) that contained a 13,896-bp ORF encoding 4,631 amino acids was obtained by RT-PCR, RACE, and bioinformatics analysis. qReal-Time PCR analysis showed that the chicken APOB gene was highly expressed in kidney, liver, and intestine. The results of differential expression showed that the APOB gene was more highly expressed in intestine and kidney in Bai'er layer than in broiler, but there was no significant difference in liver between the two breeds. The results of this study provided basic molecular information for studying the role of APOB in the energy transportation in avian species.
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