Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360838
Title PrimaTB STAT-PAK Assay, a Novel, Rapid Lateral-Flow Test for Tuberculosis in Nonhuman Primates
Author(s) Lyashchenko, K.P.; Greenwald, R.; Esfandiari, J.; Greenwald, D.; Nacy, C.A.; Gibson, S.; Didier, P.J.; Washington, M.; Szczerba, P.; Motzel, S.; Handt, L.; Pollock, J.M.; McNair, J.; Andersen, P.; Langermans, J.A.M.; Verreck, F.; Ervin, S.; Ervin, F.; McCombs, C.
Source Clinical and Vaccine Immunology 14 (2007)9. - ISSN 1556-6811 - p. 1158 - 1164.
Department(s) ASG Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) virulent mycobacterium-tuberculosis - macaca-mulatta - antigen recognition - antibody-responses - serum antibodies - skin reactivity - calmette-guerin - rhesus-monkeys - diagnosis - bovis
Abstract Tuberculosis (TB) is the most important zoonotic bacterial disease in nonhuman primates (NHP). The current diagnostic method, the intradermal palpebral tuberculin test, has serious shortcomings. We characterized antibody responses in NHP against Mycobacterium tuberculosis to identify immunodominant antigens and develop a rapid serodiagnostic test for TB. A total of 422 NHP were evaluated, including 243 rhesus (Macaca mulatta), 46 cynomolgus (Macaca fascicularis), and 133 African green (Cercopithecus aethiops sabaeus) monkeys at five collaborative centers. Of those, 50 monkeys of the three species were experimentally inoculated with M. tuberculosis. Antibody responses were monitored every 2 to 4 weeks for up to 8 months postinfection by MultiAntigen Print ImmunoAssay with a panel of 12 recombinant antigens. All of the infected monkeys produced antibodies at various levels and with different antigen recognition patterns. ESAT-6 and MPB83 were the most frequently recognized proteins during infection. A combination of selected antigens which detected antibodies in all of the infected monkeys was designed to develop the PrimaTB STAT-PAK assay by lateral-flow technology. Serological evaluation demonstrated high diagnostic sensitivity (90%) and specificity (99%). The highest rate of TB detection was achieved when the skin test was combined with the PrimaTB STAT-PAK kit. This novel immunoassay provides a simple, rapid, and accurate test for TB in NHP.
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