Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 360882
Title Identification of aCD4 T cell epitope in the pneumonia virus of mice glycoprotein and characterization of its role in protective immunity
Author(s) Claassen, E.A.W.; Bleek, G.M. van; Rychnavska, Z.S.; Groot, R.J. de; Hensen, E.J.; Tijhaar, E.
Source Virology 368 (2007)1. - ISSN 0042-6822 - p. 17 - 25.
DOI https://doi.org/10.1016/j.virol.2007.06.002
Department(s) ASG Infectieziekten
Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) respiratory syncytial virus - induced immunopathology - viral-infection - th2 cells - g-protein - responses - memory - lung - proliferation - inactivation
Abstract Pneumonia virus of mice (PVM) causes bronchiolitis and pneumonia in mice. Infection is associated with high levels of viral replication in the lungs and results in the functional inactivation of pulmonary virus-specific CD8 T cells. Due to its similarity to severe human respiratory syncytial virus (RSV) infection, PVM infection in mice has been proposed as an alternative RSV model. Here, we have delineated the minimal requirements for protective T cell immunity in the PVM model. Immunization with a CD8 T cell epitope from the PVM phosphoprotein P, combined with the ovalbumin (OVA) CD4 T cell epitope, did not confer protective immunity against lethal PVM challenge, suggesting a possible role of cognate CD4 T cell immunity. To determine the role of PVM-specific CD4 T cell responses, we mapped a PVM CD4 T cell epitope in the glycoprotein G, using a panel of overlapping peptides. Although immunization with this epitope provided some protection, solid protective immunity was only observed after immunization with a combination of the PVM-specific CD4 and CD8 T cell epitopes. Analysis of post-challenge T cell responses in immunized mice indicated that G-specific pulmonary CD4 T cells displayed a mixed Th1/Th2 phenotype, which was characterized by the presence of both IL-5 and IFN-gamma secreting cells, in the absence of overt pathology.
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