Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 362713
Title Bovine respiratory syncytial virus infection influences the impact of alpha 4- and beta(2)-integrin-mediated adhesion of peripheral blood neutrophils
Author(s) Soethout, E.C.; Antonis, A.F.G.; Ulfman, L.H.; Hoek, A. van; Most, R.G. van der; Muller, K.E.; Rutten, V.
Source Clinical and Experimental Immunology 138 (2004)3. - ISSN 0009-9104 - p. 388 - 395.
DOI https://doi.org/10.1111/j.1365-2249.2004.02639.x
Department(s) ID - Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) endothelial-cells - tnf-alpha - leukocyte transmigration - pasteurella-haemolytica - pulmonary inflammation - flow conditions - ifn-gamma - integrin - migration - deficiency
Abstract Neutrophil migration into the airways and pulmonary tissue is a common finding in bovine respiratory syncytial virus (BRSV) infections. Although neutrophil trans-endothelial migration in general depends on ß2-integrins, alternative integrins such as the ¿4-integrins have been implicated. In this study, rolling and firm adhesion of peripheral blood neutrophils isolated from healthy and BRSV-infected calves to tumour necrosis factor (TNF)-¿ activated pulmonary endothelium was investigated under flow conditions in vitro. For neutrophils obtained from healthy animals, inhibition of the ß2-integrin reduced firm adhesion to 63% and inhibition of ¿4-integrin to 73% compared with untreated controls. Inhibition of both integrins reduced firm adhesion to 25%. Rolling velocity, which is used as a parameter for integrin involvement in neutrophil rolling, increased 1·7-fold by blocking ß2-integrin and was significantly augmented to 2·5-fold by blocking both ¿4- and ß2-integrins. For neutrophils obtained from BRSV-infected animals, however, rolling velocities at 10 days after infection (p.i.) were not influenced by blocking adhesion of ¿4- and ß2-integrins, indicating that these integrins did not support neutrophil rolling. In addition, the inhibition of firm adhesion by blocking both ¿4- and ß2-integrins was reduced significantly 9 days post-infection, resulting in a residual 68% neutrophil binding at 9 days p.i. Non-blocked firm adherence was not reduced, indicating that binding was achieved by other mechanisms than through ¿4- and ß2-integrins. These results demonstrate an important function for ¿4- and ß2-integrins in rolling and firm adherence of bovine neutrophils, to TNF-¿-activated endothelium and show the dynamic use of these integrins for adhesion and migration by neutrophils in the course of BRSV infection.
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