Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 362753
Title Functional Region Identification in Proteins by Accumulative-Quantitative Peptide Mapping Using RP-HPLC-MS
Author(s) Kuipers, B.J.H.; Bakx, E.J.; Gruppen, H.
Source Journal of Agricultural and Food Chemistry 55 (2007)23. - ISSN 0021-8561 - p. 9337 - 9344.
DOI https://doi.org/10.1021/jf071380b
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) mass-spectrometry - soy proteins - absorption - allergen - acids
Abstract A new method was developed to identify regions in proteins from which peptides are derived with specific functional properties. This method is applicable for systems in which peptides of a hydrolyzed protein possess specific functional properties, but are too large to be sequenced directly and/or the peptide mixture is too complex to purify and characterize each peptide individually. In the present work, aggregating peptides obtained by proteolytic hydrolysis of soy glycinin were used as a case study. The aggregating peptides are isolated and subsequently further degraded with trypsin to result in peptides with a mass
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