Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 364698
Title Delayed senescence in cauliflower transformed with an autoregulated isopentenyl transferase gene
Author(s) Nguyen, K.H.; Jordi, W.J.R.M.; Dun, K.; Schepers, F.; Davelaar, E.; Stoopen, G.M.; Dix, P.J.; Kane, E.J.
Source International Journal of Plant Sciences 169 (2008)3. - ISSN 1058-5893 - p. 339 - 347.
DOI https://doi.org/10.1086/526466
Department(s) PRI Bioscience
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) leaf senescence - tobacco plants - cytokinin - agrobacterium - expression - cultures
Abstract Cauliflower (Brassica oleracea var. botrytis) was transformed, using Agrobacterium tumefaciens, with an autoregulated isopentenyl transferase (ipt) gene under the control of a senescence-associated gene promoter, pSAG12, isolated from Arabidopsis thaliana. The effect of introducing this chimeric construct on cytokinin (CK) content, chlorophyll retention, and plant morphology and development were investigated. A range of CK and chlorophyll contents was found among the individual primary transformants. Progeny were studied from one of the primary transformed lines that did not have elevated cytokinin content and was phenotypically similar to the parent line but displayed delayed leaf senescence. The pSAG12:ipt gene was inherited in a Mendelian manner, and the effect of this gene on senescence-related parameters was observed in a number of the progeny. While the pSAG12:ipt progeny did exhibit delayed leaf senescence, it was accompanied by undesirable agronomic traits, including less synchronous curd initiation, smaller curd size, and greater susceptibility to fungal infection.
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