Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 365589
Title Towards an optimal process for gelatinisation and hydrolysis of highly concentrated starch-water mixtures with alpha-amylase from B. licheniformis
Author(s) Baks, T.; Kappen, F.H.J.; Janssen, A.E.M.; Boom, R.M.
Source Journal of Cereal Science 47 (2008)2. - ISSN 0733-5210 - p. 214 - 225.
DOI https://doi.org/10.1016/j.jcs.2007.03.011
Department(s) Food Process Engineering
AFSG Biobased Products
Onderwijsinstituut
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) twin-screw extruder - wheat-starch - enzymatic-hydrolysis - extrusion-cooking - corn starch - sago starch - model - liquefaction - degradation - kinetics
Abstract The enzymatic hydrolysis of starch is usually carried out with 30¿35 w/w% starch in water. Higher substrate concentrations (50¿70 w/w%) were reached by using a twin-screw extruder for gelatinisation and for mixing enzyme with gelatinised starch prior to enzymatic hydrolysis in a batch reactor. The aim of this study was to determine which parameters are important for gelatinisation of wheat starch and to investigate the effects of different extrusion conditions on the enzymatic hydrolysis. After extrusion, the degree of gelatinisation was measured. During hydrolysis, the carbohydrate composition, the dextrose equivalent (DE) and the alpha-amylase activity were measured. Gelatinisation measurements showed that mechanical forces lowered the temperature required for complete gelatinisation. During hydrolysis experiments, high DEs were observed even if starch was not completely gelatinised during extrusion. Due to high substrate concentrations, the residual alpha-amylase activity remained high throughout enzymatic hydrolysis, although high temperatures were used. Increased substrate concentrations did not affect the carbohydrate composition of the product. Furthermore, the time required for the batch hydrolysis step could be varied by choosing a different enzyme-to-substrate ratio. This article provides a basis for detailed optimisation of this process to develop an industrial-scale process at high substrate concentrations.
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