Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 365847
Title Massetolide A biosynthesis in Pseudomonas fluorescens
Author(s) Bruijn, I. de; Kock, M.J.D. de; Waard, P. de; Beek, T.A. van; Raaijmakers, J.M.
Source Journal of Bacteriology 190 (2008)8. - ISSN 0021-9193 - p. 2777 - 2789.
DOI https://doi.org/10.1128/JB.01563-07
Department(s) Laboratory of Phytopathology
Nursery Stock-Flower Bulbs
Biophysics
Laboratory for Organic Chemistry
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) syringae pv.-syringae - nonribosomal peptide-synthesis - n-acylhomoserine lactones - quorum-sensing system - gene-cluster - biofilm formation - putisolvin-ii - arthrofactin synthetase - adenylation domains - mutational analysis
Abstract Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes, designated massA, massB, and massC, spanning approximately 30 kb. Prediction of the nature and configuration of the amino acids by in silico analysis of adenylation and condensation domains of the NRPSs was consistent with the chemically determined structure of the peptide moiety of massetolide A. Structural analysis of massetolide A derivatives produced by SS101 indicated that most of the variations in the peptide moiety occur at amino acid positions 4 and 9. Regions flanking the mass genes contained several genes found in other Pseudomonas CLP biosynthesis clusters, which encode LuxR-type transcriptional regulators, ABC transporters, and an RND-like outer membrane protein. In contrast to most Pseudomonas CLP gene clusters known to date, the mass genes are not physically linked but are organized in two separate clusters, with massA disconnected from massB and massC. Quantitative real-time PCR analysis indicated that transcription of massC is strongly reduced when massB is mutated, suggesting that these two genes function in an operon, whereas transcription of massA is independent of massBC and vice versa. Massetolide A is produced in the early exponential growth phase, and biosynthesis appears not to be regulated by N-acylhomoserine lactone-based quorum sensing. Massetolide A production is essential in swarming motility of P. fluorescens SS101 and plays an important role in biofilm formation.
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