Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 365893
Title Synergy of DNA-bending nucleoid proteins and macromolecular crowd-condensing DNA
Author(s) Bessa Ramos, E.; Wintraecken, C.H.J.M.; Geerling, A.C.M.; Vries, R.J. de
Source Biophysical Reviews and Letters 2 (2007)3/4. - ISSN 1793-0480 - p. 259 - 265.
DOI https://doi.org/10.1142/S1793048007000556
Department(s) Physical Chemistry and Colloid Science
Microbiological Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2007
Abstract Many prokaryotic nucleoid proteins bend DNA and form extended helical protein-DNA fibers rather than condensed structures. On the other hand, it is known that such proteins (such as bacterial HU) strongly promote DNA condensation by macromolecular crowding. Using theoretical arguments, we show that this synergy is a simple consequence of the larger diameter and lower net charge density of the protein-DNA filaments as compared to naked DNA, and hence, should be quite general. To illustrate this generality, we use light-scattering to show that the 7kDa basic archaeal nucleoid protein Sso7d from Sulfolobus solfataricus (known to sharply bend DNA) likewise does not significantly condense DNA by itself. However, the resulting protein-DNA fibers are again highly susceptible to crowding-induced condensation. Clearly, if DNA-bending nucleoid proteins fail to condense DNA in dilute solution, this does not mean that they do not contribute to DNA condensation in the context of the crowded living cell.
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