Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 365976
Title Improved postthaw viability and in vitro functionality of peripheral blood hematopoietic progenitor cells after cryopreservation with a theoretically optimized freezing curve
Author(s) Tijssen, M.R.; Woelders, H.; Vries - van Rossen, A. de; Schoot, C. van der; Voermans, C.; Lagerberg, J.W.M.
Source Transfusion 48 (2008)5. - ISSN 0041-1132 - p. 893 - 901.
DOI https://doi.org/10.1111/j.1537-2995.2008.01650.x
Department(s) Livestock Research
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) cd34(+) cells - clinical toxicity - stem-cells - apoptosis - permeability - infusion - grafts - marrow - death
Abstract The freezing curve currently used for the cryopreservation of peripheral blood stem cell transplants (PBSCTs) has been determined empirically. Although the use of cryopreserved PBSCTs is successful and usually leads to rapid hematopoietic recovery, the freeze-thawing process is known to induce a significant degree of cell death. Furthermore, the infusion of dimethyl sulfoxide (DMSO), used to protect the cells against damage induced by freezing, can cause morbidity. Therefore, optimizing the current cryopreservation protocol (with 10% DMSO and a slow linear cooling curve) with theoretically optimized freezing curves and a lower DMSO concentration might improve the recovery after transplantation
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