Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 367686
Title CE-MSn of complex pectin-derived oligomers
Author(s) Coenen, G.J.; Kabel, M.A.; Schols, H.A.; Voragen, A.G.J.
Source Electrophoresis 29 (2008)10. - ISSN 0173-0835 - p. 2101 - 2111.
DOI https://doi.org/10.1002/elps.200700465
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) anion-exchange chromatography - capillary zone electrophoresis - trap mass-spectrometry - hairy ramified regions - maldi-tof ms - rhamnogalacturonan-i - 8-aminonaphthalene-1,3,6-trisulfonic acid - galacturonic acid - apple pectin - oligosaccharides
Abstract As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high-performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE-IT-MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low-pH CE-LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE-MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE-MSn. Oligomers present in low amounts could be localized by their corresponding m/z, as was demonstrated by selected mass range representation.
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