Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 368738
Title Thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum
Author(s) Trifonova, R.D.; Postma, J.; Ketelaars, J.J.M.H.; Elsas, J.D. van
Source Microbial Ecology 56 (2008)3. - ISSN 0095-3628 - p. 561 - 571.
DOI https://doi.org/10.1007/s00248-008-9376-9
Department(s) Biointeracties and Plant Health
Agrosystems
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) coconut coir dusts - sp-nov. - soil - methylobacterium - 4-chlorophenol - microorganisms - phytotoxicity - populations - sequence - removal
Abstract This study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. Torrefied grass fibers (TGF) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. TGF and torrefied grass extracts (TGE) were inoculated with a suspension of microorganisms obtained from soil. Sequential microbial enrichment steps were then performed in both substrates. The microbial communities developing in the substrates were assessed using cultivation-based and cultivation-independent approaches. Thus, bacterial isolates were obtained, and polymerase chain reaction¿denaturing gradient gel electrophoresis (DGGE) analyses for bacterial communities were performed. Partial sequencing of the 16S ribosomal RNA gene from isolates and bands from DGGE gels showed diverse communities after enrichment in TGE and TGF. Bacterial isolates affiliated with representatives of the ¿-proteobacteria (Methylobacterium radiotolerans, Rhizobium radiobacter), ¿-proteobacteria (Serratia plymuthica, Pseudomonas putida), Cytophaga¿Flavobacterium¿Bacteroides (CFB) group (Flavobacterium denitrificans), ß-proteobacteria (Ralstonia campinensis), actinobacteria (Cellulomonas parahominis, Leifsonia poae, L. xyli subsp. xyli, and Mycobacterium anthracenicum), and the firmicutes (Bacillus megaterium) were found. In TGE, ¿-proteobacteria were dominant (61.5% of the culturable community), and 20% belonged to the CFB group, whereas actinobacteria (67.4%) and ¿-proteobacteria (21.7%) were prevalent in TGF. A germination assay with lettuce seeds showed that the phytotoxicity of TGF and TGE decreased due to the microbial enrichment
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