Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 369406
Title Cloning of a prolidase gene from Aspergillus nidulans and characterisation of its product
Author(s) Jalving, R.; Bron, P.; Kester, H.C.M.; Visser, J.; Schaap, P.J.
Source Molecular Genetics and Genomics 267 (2002)2. - ISSN 1617-4615 - p. 218 - 222.
Department(s) Microbiological Laboratory
FB Inkoop
AFSG Staff Departments (WUATV)
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) escherichia-coli - transformation - aminopeptidase - purification - expression - peptides
Abstract Using EST sequence information available from the filamentous fungus Aspergillus nidulans as a starting point, we have cloned the prolidase-encoding gene, designated pepP. Introduction of multiple copies of this gene into the A. nidulans genome leads to overexpression of an intracellular prolidase activity. Prolidase was subsequently purified and characterised from an overexpressing strain. The enzyme activity is dependent on manganese as a cofactor, is specific for dipeptides and hydrolyses only dipeptides with a C-terminal proline residue. Although these proline dipeptides are released both intracellularly and extracellularly, prolidase activity was detected only intracellularly
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