Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 369640
Title Role of cyclic lipopeptide surfactants in bacterial defense against protozoan predation
Author(s) Mazzola, M.; Raaijmakers, J.M.
Source In: APS Centennial Meeting, Minneapolis, Minnesota, USA, 26-30 July 2008 - p. S100 - S100.
Department(s) Laboratory of Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2008
Abstract Cyclic lipopeptides (CLPs) are produced by a diversity of bacterial genera, possess biosurfactant activity, and play a key role in a multiplicity of natural functions for the producing bacteria, including swarming motility and biofilm formation. CLPs have the capacity to disrupt membrane integrity leading to the cell lysis of certain microbial life stages, including oomycete zoospores. This study shows that the CLPs massetolide A and viscosin produced by the bacteria Pseudomonas fluorecens strains SS101 and SBW25 lead to lysis of protozoan trophozoites and confer protection from predation by the amoebaflagellate Naegleria americana. In vitro, massetolide A-producing SS101 and viscosin-producing SBW25 were significantly less susceptible to grazing by N. americana than the corresponding CLP-deficient massA mutant 10.24 and viscA mutant of SBW25. Genetic complementation of the massA mutation in 10.24 restored massetolide A biosynthesis and protection from N. americana predation. Populations of mutant 10.24 exhibited a more rapid decline in bulk soil relative to the parental strain, with SS101 maintaining a population approximately one order of magnitude greater than the surfactant-deficient mutant two weeks post-inoculation. Collectively these results show, for the first time, that CLPs produced by Pseudomonas contribute to survival in soil and are potent metabolites in the bacterial defense against protozoan predation
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