Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 370267
Title The role of ornithine aminotransferase in fruiting body formation of the mushroom Agaricus bisporus
Author(s) Wagemaker, M.J.M.; Eastwood, D.C.; Welagen, J.; Drift, C. van der; Jetten, M.S.M.; Burton, K.; Griensven, L.J.L.D. van; Camp, H.J.M. op den
Source Mycological Research 111 (2007)8. - ISSN 0953-7562 - p. 909 - 918.
Department(s) PRI Bioscience
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) aspergillus-nidulans - cultivated mushroom - saccharomyces-cerevisiae - serine proteinase - gene encodes - lange imbach - enzymes - repression - arginase - mycelium
Abstract The complete oat gene and cDNA from the commercial mushroom, Agaricus bisporus, encoding ornithine aminotransferase (OAT) was characterized. The gene encodes a 466 amino acid protein and provides the first fully reported homobasidiomycete OAT protein sequence. The gene is interrupted by ten introns, and no mitochondrial targeting motif was present pointing to a cytoplasmic localization. The function of the gene was demonstrated by complementation of a Saccharomyces cerevisiae mutant unable to utilize ornithine as a sole source of nitrogen with an A. bisporus oat cDNA construct. Northern analysis of the oat gene together with the pruA gene (encoding ¿1-pyrroline-5-carboxylate dehydrogenase) showed that transcripts of both genes were lower during the first stages of fruiting body development. The higher expression of the oat gene in later stages of development, suggests the importance of ornithine metabolism for the redistribution of metabolites in the developing mushroom. Hplc analysis of all amino acids revealed that ornithine levels increased during fruiting body development whereas proline levels fell
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