Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 370277
Title Early and late pathogenesis of natural scrapie infection in sheep
Author(s) Keulen, L.J.M. van; Vromans, M.E.W.; Zijderveld, F.G. van
Source Apmis 110 (2002)1. - ISSN 0903-4641 - p. 23 - 32.
Department(s) CIDC - Divisie Bacteriologie en TSE's
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) prion protein - immunohistochemical detection - spongiform encephalopathy - early accumulation - nervous-system - prp - bse - brain - mice - immunodetection
Abstract The pathogenesis of scrapie infection was studied in sheep carrying the PrPVRQ/PrPVRQ genotype, which is associated with a high susceptibility for natural scrapie. The sheep were killed at sequential time points during a scrapie infection covering both the early and late stages of scrapie pathogenesis. Various lymphoid and neural tissues were collected and immunohistochemically examined for the presence of the scrapie-associated prion protein PrPSc, a marker for scrapie infectivity. The first stage of scrapie infection consisted of invasion of the palatine tonsil and Peyer's patches of the caudal jejunum and ileum, the so-called gut-associated lymphoid tissues (GALT). At the same time, PrPSc was detected in the medial retropharyngeal lymph nodes draining the palatine tonsil and the mesenteric lymph nodes draining the jejunal and ileal Peyer's patches. From these initial sites of scrapie replication, the scrapie agent disseminated to other non-GALT-related lymphoid tissues. Neuroinvasion started in the enteric nervous system followed by retrograde spread of the scrapie agent via efferent parasympathetic and sympathetic nerve fibres innervating the gut, to the dorsal motor nucleus of the vagus in the medulla oblongata and the intermediolateral column of the thoracic spinal cord segments T8¿T10, respectively
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