Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371102
Title Osmotic shrinkage and reswelling of giant vesicles composed of dioleoylphosphatidylglycerol and cholesterol
Author(s) Claessens, M.M.A.E.; Leermakers, F.A.M.; Hoekstra, F.A.; Cohen Stuart, M.A.
Source Biochimica et Biophysica Acta. Biomembranes 1778 (2008)4. - ISSN 0005-2736 - p. 890 - 895.
Department(s) Physical Chemistry and Colloid Science
Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) isolated plant-protoplasts - plasma-membrane - lipid-bilayers - phospholipid-vesicles - mechanical-properties - cold-acclimation - freezing-injury - model membranes - curvature - stability
Abstract The osmotic shrinkage of giant unilamellar dioleoylphosphatidylglycerol (DOPG) vesicles in a hypertonic osmotic solution is investigated. The volume reduction for given membrane area leads to a vesiculation of the bilayer into the interior of the giant. The size of the daughter vesicles that appear inside the giant is uniform and an increasing function of the cholesterol content, but independent of the osmotic gradient applied. The radius of the daughter vesicles increases from 0.2 mum to 3.0 mum when the cholesterol content is changed from 0 to 40%. It is argued that the size of the daughter vesicles is regulated by the membrane persistence length, which is an exponential function of the mean bending modulus. From the kinetics of shrinkage it follows that approximately 14% of the daughter vesicles remain attached to the mother giant. This is in reasonable agreement with osmotic swelling experiments which show that approximately 11% of the daughter vesicles is available for area expansion.
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