Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371323
Title Cyprinid gonad differentiation: A comparative genomic study
Author(s) Bartfai, R.; Wang, X.; Cai, M.; Srinivasan, H.; Hossain, M.S.; Komen, J.; Christoffels, A.; Orban, L.
Source Aquaculture 272 (2007)1. - ISSN 0044-8486 - p. s244 - s245.
DOI https://doi.org/10.1016/j.aquaculture.2007.07.032
Department(s) Animal Breeding and Genetics
WIAS
Publication type Abstract in scientific journal or proceedings
Publication year 2007
Abstract We study the molecular regulation of sex in cyprinids, with special emphasis on male gonad differentiation by using the tools of comparative and functional genomics. We have clustered nearly 60 thousand gonad-derived zebrafish ESTs to yield over 6500 ¿Gonad Uniclone¿ sequences (over 1500 unique to our collection). Inserts of these selected clones were printed onto slides to produce the first gonad-derived cDNA microarray from zebrafish. Labeled RNAs from adult and differentiating gonads were hybridized onto the array to identify differentially expressed genes. Several of these genes are novel and conserved among vertebrates, the preliminary analysis of a selected set together with candidate genes will be presented. The development of vasegfp transgenic individuals (from Lisbeth Olsen, Bergen, Norway) was followed by detecting the level of EGFP expression in their gonads. Samples collected at selected time points were subjected to histological analysis. Our data indicate that all zebrafish males go through an ovary-to-testis transformation, however variable starting time and wide-ranging levels of EGFP expression in ¿juvenile ovaries¿ yield a complex picture even among siblings. Differentiating testis samples were isolated from monosex common carp individuals produced by crossing YY ¿supermale¿ individuals with XX females between 60 and 100 days post fertilization. Clustered cDNA sequences were generated and compared to the zebrafish ¿Gonad Uniclone¿ cDNA set to identify novel clones expressed very early during testis formation. When mapped to the zebrafish genome common carp cDNAs identified a number of novel genes, which were not predicted by either experimental or bioinformatic methods.
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