Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371511
Title Reduction of protein adsorption to a solid surface by a coating composed of polymeric micelles with a glass-like core
Author(s) Hofs, P.S.; Brzozowska, A.M.; Keizer, A. de; Norde, W.; Cohen Stuart, M.A.
Source Journal of Colloid and Interface Science 325 (2008)2. - ISSN 0021-9797 - p. 309 - 315.
DOI https://doi.org/10.1016/j.jcis.2008.06.006
Department(s) Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) atomic-force microscopy - block-copolymers - assembled monolayers - polyethylene oxide - kinetics - brushes - silica - resistance - fibrinogen - interface
Abstract Adsorption studies by optical reflectometry show that complex coacervate core micelles (C3Ms) composed of poly([4-(2-amino-ethylthio)-butylene] hydrochloride)49-block-poly(ethylene oxide)212 and poly([4-(2-carboxy-ethylthio)-butylene] sodium salt)47-block-poly(ethylene oxide)212 adsorb in equal amounts to both silica and cross-linked 1,2-polybutadiene (PB). The C3Ms have an almost glass-like core and atomic force microscopy of a dried layer of adsorbed C3Ms shows densely packed flattened spheres on silica, which very probably are adsorbed C3Ms. Experiments were performed with different types of surfaces, solvents, and proteins; bare silica and cross-linked 1,2-PB, NaNO3 and phosphate buffer, and lysozyme, bovine serum albumin, ß-lactoglobulin, and fibrinogen. On the hydrophilic surface the coating reduces protein adsorption >90% in 0.1 M phosphate buffer, whereas the reduction on the coated hydrophobic surface is much lower. Reduction is better in phosphate buffer than in NaNO3, except for the positively charged lysozyme, where the effect is reversed.
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