Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371589
Title Immunohistochemical features of Prp(d) accumulation in natural and experimental goat transmissible spongiform encephalopathies
Author(s) Jeffrey, M.; Martin, S.; Gonzalez, L.; Foster, J.; Langeveld, J.P.M.; Zijderveld, F.G. van; Grassi, J.; Hunter, N.
Source Journal of Comparative Pathology 134 (2006)2-3. - ISSN 0021-9975 - p. 171 - 181.
DOI https://doi.org/10.1016/j.jcpa.2005.10.003
Department(s) CIDC - Divisie Bacteriologie en TSE's
CVI - Divisie Bacteriologie en TSE's
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) disease-associated prp - prion protein - monoclonal-antibodies - infected sheep - host genotype - amino-acid - bse agent - scrapie - brain - mice
Abstract Scrapie is a transmissible spongiform encephalopathy (TSE) or prion disease, which naturally affects sheep and goats. Immunohistochemical epitope mapping of abnormal PrP accumulations (PrPd) in brain can help in characterizing sheep TSE sources or strains and in identifying potential bovine spongiform encephalopathy (BSE) infections of sheep. Natural and experimental TSE infections of goats were examined to determine whether the epitope mapping approach could also be applied to aid recognition of BSE infection in goats. Goats experimentally infected with the SSBP/1 or CH1641 sheep scrapie strains or with cattle BSE, together with four field cases of natural TSE in goats, were examined immunohistochemically with six different antibodies. CH1641 and SSBP/1 infections in goats, as in sheep, showed PrPd accumulations which were mainly intracellular. Some differences in targeting, particularly of Purkinje cells, was evident in inter-species comparisons of CH1641 and SSBP/1. PrPd labelling of goat BSE experimental cases showed extensive intracellular and extracellular accumulations, also similar to those in sheep BSE. Intra-neuronal PrPd in both goat and sheep BSE was labelled only by antibodies recognizing epitopes located C-terminally of residue His99, whereas in natural sheep TSE sources, and in sheep and goat SSBP/1, PrPd was also detected by antibodies to epitopes located between residues Trp93 and His99. Testing of four natural goat TSE samples showed one case in which epitope mapping characteristics and the overall patterns of PrPd accumulation was identical with those of experimental goat BSE. The four natural goat scrapie cases examined showed some degree of immunohistochemical phenotype variability, suggesting that multiple strains exist within the relatively small UK goat population.
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