Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371887
Title AtSERK1 expression precedes and coincides with early somatic embryogenesis in Arabidopsis thaliana
Author(s) Salaj, J.; Recklinghausen, I.R. von; Hecht, V.; Vries, S.C. de; Schel, J.H.N.; Lammeren, A.A.M. van
Source Plant Physiology and Biochemistry 46 (2008)7. - ISSN 0981-9428 - p. 709 - 714.
DOI https://doi.org/10.1016/j.plaphy.2008.04.011
Department(s) Biochemistry
Laboratory of Cell Biology
EPS
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) arabinogalactan proteins - developmental pathway - tissue cultures - callus-cultures - cell - gene - embryos - carrot - plants - methacrylate
Abstract The Arabidopsis thaliana primordia timing (pt) mutant was transformed with an AtSERK1::GUS construct. Liquid cultures of this line were used to study the relationship between somatic embryogenesis and the expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (AtSERK1) as a marker for cells competent to form embryos. In order to search for the expression of AtSERK1::GUS during early stages of somatic embryogenesis, histochemical as well as immunochemical approaches were used for the detection of ß-glucuronidase (GUS). Four sites of AtSERK1 expression were found in the embryogenic cultures: in embryogenic callus, where primary somatic embryos developed; in the basal parts of primary somatic embryos; in the outer layers of cotyledons of primary somatic embryos where secondary embryos were formed; and in provascular and vascular strands of developing somatic embryos. The in vitro expression of AtSERK1::GUS coincides with embryogenic development up to the heart-shaped stage. Prior to the expression in embryos, AtSERK1 was expressed in single cells and small cell clusters, indicating that AtSERK1 indeed marks embryogenic competence. Its expression in (pro)
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