Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371954
Title Development of an automated system for isolation and purification of humic substances
Author(s) Zomeren, A. van; Weij-Zuiver, E. van der; Comans, R.N.J.
Source Analytical and Bioanalytical Chemistry 391 (2008)6. - ISSN 1618-2642 - p. 2365 - 2370.
DOI https://doi.org/10.1007/s00216-008-2163-0
Department(s) Sub-department of Soil Quality
Chair Soil Chemistry and Chemical Soil Quality
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) preparative isolation - natural-waters
Abstract Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system lies in the way the multiple liquids and columns used in the isolation/purification procedure are handled in both forward and back-elution mode by solenoid valves. The automated procedure significantly reduces the total throughput time needed, from 6¿7 days to 48 h, and the amount of labor to obtain purified HS for further characterization. Chemical characterization of purified HS showed that results were in good agreement with previously published values for HS from a variety of sources, including the IHSS standard HS collection. It was also shown that the general properties of HS were consistent among the different source materials (soil, waste, aquatic) used in this study. The developed system greatly facilitates isolation and characterization of HS and reduces the risk of potential (time-dependent) alteration of HS properties in the manual procedure
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