Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371989
Title Conservation of DNA photolyase genes in plusiine nucleopolyhedroviruses
Author(s) Xu, F.; Vlak, J.M.; Oers, M.M. van
Source In: Abstract book of the 41st Annual Meeting of the Society for Invertebrate Pathology and 9th International Conference on Bacillus thuringiensis, Warwick, United Kingdom, 3 - 7 August, 2008. - Warwick : University of Warwick - p. 75 - 75.
Event Warwick : University of Warwick 41st Annual Meeting of the Society for Invertebrate Pathology and 9th International Conference on Bacillus thuringiensis, 2008-08-03/2008-08-07
Department(s) Laboratory of Virology
PE&RC
Publication type Abstract in scientific journal or proceedings
Publication year 2008
Abstract DNA photolyase genes (phr) encode photoreactive enzymes, which are involved in the repair of UV-damaged DNA. Cyclobutane pyrimidine dimer (CPD) specific photolyase genes are present in nucleopolyhedroviruses isolated from Chrysodeixis chalcites (ChchNPV) and Trichoplusia ni (TnSNPV) insects belonging to the Plusiinae subfamily (Noctuidae). To better understand the occurrence and evolution of these genes in baculoviruses, we investigated their possible conservation in other group II NPVs, which infect plusiine insects. A PCR based strategy using degenerate phr-specific primers was designed and validated to detect and analyze possible photolyase genes. Six additional Plusiinae-infecting NPVs were analyzed and all but one contained one or more phr-like sequences. Thysanoplusia orichalcea NPV A28-1 appeared to be a group I NPV, and did not contain a phr homologue. Phylogenetic analysis revealed that all photolyase genes of the tested Plusiinaeinfecting baculoviruses group in a single clade. Moreover, the phylogeny of the polyhedrin sequences of these viruses confirmed that the analyzed viruses also formed a single clade in group II NPVs. We hypothesize that all plusiine group II NPVs contain one or more photolyase genes and that these have a common ancestor. The correlation between baculoviruses having phr genes and the behaviour of their plusiine hosts will be discussed.
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