Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 372249
Title Demonstration of In vivo expression of a hypothetical open reading frame (ORF-14) encoded by the RD1 region of Mycobacterium tuberculosis
Author(s) Amoudy, H.A.; Ahmad, S.; Thole, J.E.R.; Mustafa, A.S.
Source Scandinavian Journal of Immunology 66 (2007)4. - ISSN 0300-9475 - p. 422 - 425.
Department(s) ASG Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) t-cell responses - calmette-guerin - genome sequence - esat-6 antigen - identification - proteins - epitopes - bcg - immunogenicity - compilation
Abstract Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.
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