Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 372673
Title Enzymatically-tailored pectins differentially influence the morphology, adhesion, cell cycle progression and survival of fibroblasts
Author(s) Nagel, M.D.; Verhoef, R.P.; Schols, H.A.; Morra, M.; Knox, J.P.; Ceccone, G.; Volpe, C.D.; Vigneron, P.; Bussy, C.; Gallet, M.; Velzenberger, E.; Vayssade, M.; Cascardo, G.; Cassinelli, C.; Haeger, A.; Gilliland, D.; Liakos, I.; Rodrigues-Valverde, M.; Siboni, S.
Source Biochimica et Biophysica Acta. General subjects 1780 (2008)7-8. - ISSN 0304-4165 - p. 995 - 1003.
DOI https://doi.org/10.1016/j.bbagen.2008.04.002
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) hairy ramified regions - growth-factor receptors - surface-chemistry - fibronectin conformation - arabinogalactan-proteins - monoclonal-antibodies - human keratinocytes - integrin binding - adsorbed fibronectin - rhamnogalacturonan-i
Abstract Improved biocompatibility and performance of biomedical devices can be achieved through the incorporation of bioactive molecules on device surfaces. Five structurally distinct pectic polysaccharides (modified hairy regions (MHRs)) were obtained by enzymatic liquefaction of apple (MHR-B, MHR-A and MHR-), carrot (MHR-C) and potato (MHR-P) cells. Polystyrene (PS) Petri dishes, aminated by a plasma deposition process, were surface modified by the covalent linking of the MHRs. Results clearly demonstrate that MHR-B induces cell adhesion, proliferation and survival, in contrast to the other MHRs. Moreover, MHR- causes cells to aggregate, decrease proliferation and enter into apoptosis. Cells cultured in standard conditions with 1% soluble MHR-B or MHR- show the opposite behaviour to the one observed on MHR-B and --grafted PS. Fibronectin was similarly adsorbed onto MHR-B and tissue culture polystyrene (TCPS) control, but poorly on MHR-. The Fn cell binding site (RGD sequence) was more accessible on MHR-B than on TCPS control, but poorly on MHR-. The disintegrin echistatin inhibited fibroblast adhesion and spreading on MHR-B-grafted PS, which suggests that MHRs control fibroblast behaviour via serum-adhesive proteins. This study provides a basis for the design of intelligently-tailored biomaterial coatings able to induce specific cell functions.
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