Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 372676
Title A 15N stable isotope semen label to detect mating in the malaria mosquito Anopheles arabiensis Patton
Author(s) Helinski, M.; Hood-Nowotny, R.C.; Gludovacz, D.; Mayr, L.; Knols, B.G.J.
Source Parasites & Vectors 1 (2008). - ISSN 1756-3305 - 4 p.
DOI https://doi.org/10.1186/1756-3305-1-19
Department(s) Laboratory of Entomology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2008
Abstract In previous studies it was determined that the stable isotope 13-carbon can be used as a semen label to detect mating events in the malaria mosquito Anopheles arabiensis. In this paper we describe the use of an additional stable isotope, 15-nitrogen (15N), for that same purpose. Both stable isotopes can be analysed simultaneously in a mass spectrometer, offering the possibility to detect both labels in one sample in order to study complex and difficult-to-detect mating events, such as multiple mating. 15N-glycine was added to larval rearing water and the target enrichment was 5 atom% 15N. Males from these trays were mated with unlabelled virgin females, and spiked spermathecae were analysed for isotopic composition after mating using mass spectrometry. Results showed that spermathecae positive for semen could be distinguished from uninseminated or control samples using the raw ¿15N¿ values. The label persisted in spermathecae for up to 5 days after insemination, and males aged 10 days transferred similar amounts of label as males aged 4 days. There were no negative effects of the label on larval survival and male longevity. Enrichment of teneral mosquitoes after emergence was 4.85 ± 0.10 atom% 15N. A threshold value defined as 3 standard deviations above the mean of virgin (i.e. uninseminated spermathecae) samples was successful in classifying a large proportion of samples correctly (i.e. on average 95%). We conclude that alongside 13C, 15N can be used to detect mating in Anopheles and the suitability of both labels is briefly discussed.
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