Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 372893
Title Interplay between macrophages and adipocytes: stimulation of secretion of adipokines and the role of endocannabinoids
Author(s) Wortelboer, H.M.; Lancee, C.A.A.; Meijerink, J.; Witkamp, R.F.
Source International Journal of Obesity 32 (2008)S1. - ISSN 0307-0565 - p. S64 - S64.
Department(s) Chair Nutrition Metabolism and Genomics
Chair Nutrition and Pharmacology (HNE)
Publication type Abstract in scientific journal or proceedings
Publication year 2008
Abstract The concept of a chronic low-grade inflammatory status of adipose tissue during obesity is now widely accepted. This condition is regarded as a major driver for the development of cardiovascular- and diabetic complications. Macrophage recruitment and -activation is pivotal for the inflammatory process, and the different cell types and forms are involved in a complex interplay. We are investigating the regulation of intercellular communication, with special emphasis on the role of cannabinoid type-1 receptor (CB1) and CB2 mediated pathways. In order to study the effects of macrophage-secreted factors on adipocytes, 3T3-L1 adipocytes were incubated with lipopolysaccharide (LPS) or different RAW264.7 macrophage conditioned media. The release of adipokines by 3T3 cells was measured by multiplex immunoassay. Macrophage-LPS stimulated conditioned medium enhanced the adipokine secretion of especially MCP-1, PAI-1 and adiponectin, whereas release of IL-6 was lower as compared to LPS treatment. No effect on TNF-a secretion was observed. Moreover, an enhanced secretion of MCP-1 and PAI-1, but not IL-6, was measured after treatment with macrophage-stimulated conditioned medium (in absence of LPS). Macrophage conditioned medium but not LPS also increased lipolysis in 3T3-L1 cells. When studying the effects of CB1 and CB2 agonists on macrophages and adipocytes, no effects were seen so far on the TNF-a, IL-6 or IL10 secretion by macrophages stimulated with LPS. We are currently studying other ways of macrophage activation. By contrast, the cannabinoid agonist HU210 induced a 3-fold induction of triglyceride content without affecting adiponectin mRNA levels.
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