Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 387300
Title Biomarker development for recovery from stress in pig muscles
Author(s) Pas, M.F.W. te; Keuning, E.; Kruijt, L.; Wiel, D.F.M. van de; Young, J.F.; Oksbjerg, N.
Source In: Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. - Wageningen Academic Publishers - ISBN 9789086861217 - p. 279 - 279.
Event 60th Annual Meeting of the European Association for Animal Production, 2009-08-24/2009-08-27
Department(s) Livestock Research
LR - Backoffice
Publication type Abstract in scientific journal or proceedings
Publication year 2009
Abstract Pork quality is negatively influenced by stress due to pre-slaughter exhaustion of energy metabolism components in muscle fibres. Changes in the proteome are expected due to related protein breakdown. Thus proteome profiles may indicate the status of stress and recovery from stress of muscle tissue. Therefore, pigs were exercised on a treadmill for 30 minutes and slaughtered immediately or after a recovery period of 1 or 3 hours. Control pigs were treated as regular slaughter pigs. Each group consisted of 10 pigs. The longissimus and biceps femoris muscles were sampled. Proteomics profiles were determined for each individual sample using the SELDI-TOF-MS equipment and 3 different array types analyzing positively or negatively charged proteins, or phosphorylated proteins, respectively. Analyses were performed with the software provided with the equipment. The analyses compared the expression levels of individual peaks for each of the animals per muscle and per treatment group. All proteomics experiments were done in duplicate. Each proteomics experiment was individually analyzed and only peaks that appeared in both analyses were considered significant. The first results indicate that the changes of the expression of a number of peaks may be related to stress and recovery of stress. Compared to non-stressed control pigs the expression of these proteins is either decreased or elevated after stress and the changes are reversed during the recovery period. However, peaks differ in the timing of this process. Therefore, at present we cannot conclude on the association between peak expression profile and stress recovery. Further analyses on the repeatability of the analyses are ongoing. Finally, it may be necessary to associate the proteomics expression profiles with physiological indicators of muscle response to stress before conclusions about possible biomarkers can be done.
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