Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 388158
Title Replication of Agrotis segetum granulovirus in continuous insect cell lines
Author(s) Lynn, D.R.; Lent, J.W.M. van; Oers, M.M. van; Vlak, J.M.
Source In: Abstract Book of the 42nd Annual Meeting of the Society for Invertebrate Pathology, Park City, Utah, USA, 16 - 20 August, 2009. - Park City, Utah : - p. 55 - 55.
Event Park City, Utah : 42nd Annual Meeting of the Society for Invertebrate Pathology, Park City, Utah, USA, 2009-08-16/2009-08-20
Department(s) Laboratory of Virology
PE&RC
Publication type Abstract in scientific journal or proceedings
Publication year 2009
Abstract The Agrotis ipsilon cell lines, IPLB-AiE1611T and AiEd6T, were inoculated with occlusion-body derived virions (ODV) of the A. segetum granulovirus (AgseGV). The capsules were dissolved by treatment with 0.1M NaH2CO3/0.1M NaCl for 30 min. to release the occluded virions followed by neutralization with an acidic salt solution and inoculation onto cells in 24-well tissue culture plates. Within a few days, numerous cells in the culture developed cytopathic effects consisting of darkened nuclei and enlarged cells when examined with phase contrast or Nomarski microscopy optics. After one week dense particles were seen within the nuclei of infected cells and the infection extended in the culture. Both cell lines replicated AgseGV equally well. Transfer of filtered supernatant from the ODV-inoculated culture to fresh cells led to similar cytopathic effects in the new culture suggesting the production of infectious (budded) virus from the infected cells. Electron microscopy of cells inoculated with the 2nd passage virus showed typical granulovirus capsules in the nuclei of many cells and in great numbers. The capsules are being tested for in vivo infectivity in A. segetum larvae. This is the first successful replication of this virus in cell cultures and one of the few examples of in vitro replication and passaging of granuloviruses. It opens up the possibility to study the molecular genetics and functional biology of AgseGV in much detail and to engineer this virus for better insecticidal properties. This work was made possible through a visitors grant from the Wageningen Graduate School ‘Production Ecology & Resource Conservation’
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