Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 399927
Title Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1
Author(s) Wegkamp, A.; Mars, A.E.; Faijes, M.; Molenaar, D.; Vos, R.C.H. de; Klaus, M.J.; Hanson, A.D.; Vos, W.M. de; Smid, E.J.
Source Microbial Cell Factories (2010). - ISSN 1475-2859 - p. 9:100 - 9:100.
Department(s) FBR Bioconversion
AFSG Biobased Products
PRI BIOS Applied Metabolic Systems
Microbiological Laboratory
Food Microbiology Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) lactic-acid bacteria - lactococcus-lactis - escherichia-coli - streptococcus-cremoris - growth-rate - folic-acid - expression - protein - biosynthesis - recombinant
Abstract Background Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in Lactobacillus plantarum WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells. Results Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the pool sizes of 18 metabolites differed significantly between these strains. The gene expression profile was determined for both strains in pH-regulated chemostat culture and batch culture. Apart from the expected overexpression of the 6 genes of the folate gene cluster, no other genes were found to be differentially expressed both in continuous and batch cultures. The discrepancy between the low transcriptome and metabolome response and the 25% growth rate reduction of the folate overproducing strain was further investigated. Folate production per se could be ruled out as a contributing factor, since in the absence of folate production the growth rate of the overproducer was also reduced by 25%. The higher metabolic costs for DNA and RNA biosynthesis in the folate overproducing strain were also ruled out. However, it was demonstrated that folate-specific mRNAs and proteins constitute 8% and 4% of the total mRNA and protein pool, respectively. Conclusion Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that Lactobacillus plantarum WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins.
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