Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 399928
Title A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene
Author(s) Cankar, K.; Houwelingen, A.M.M.L. van; Bosch, H.J.; Sonke, Th.; Bouwmeester, H.J.; Beekwilder, M.J.
Source FEBS Letters 585 (2011)1. - ISSN 0014-5793 - p. 178 - 182.
DOI http://dx.doi.org/10.1016/j.febslet.2010.11.040
Department(s) Laboratory of Plant Physiology
PRI BIOS Applied Metabolic Systems
PRI Bioscience
EPS
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) functional-characterization - yeast expression - nootkatone - valencene - biosynthesis - sesquiterpenes - artemisinin - synthase - cloning
Abstract Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene
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