Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 400107
Title Proteomics of industrial fungi: trends and insights for biotechnology
Author(s) Oliveira, J.M.; Graaff, L.H. de
Source Applied Microbiology and Biotechnology 89 (2011)2. - ISSN 0175-7598 - p. 225 - 237.
DOI http://dx.doi.org/10.1007/s00253-010-2900-0
Department(s) Microbiological Laboratory
Systems and Synthetic Biology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) quantitative mass-spectrometry - aspergillus-fumigatus - shotgun proteomics - trichoderma-reesei - genome sequence - saccharomyces-cerevisiae - absolute quantification - penicillium-chrysogenum - intracellular proteins - extracellular proteins
Abstract Filamentous fungi are widely known for their industrial applications, namely, the production of food-processing enzymes and metabolites such as antibiotics and organic acids. In the past decade, the full genome sequencing of filamentous fungi increased the potential to predict encoded proteins enormously, namely, hydrolytic enzymes or proteins involved in the biosynthesis of metabolites of interest. The integration of genome sequence information with possible phenotypes requires, however, the knowledge of all the proteins in the cell in a system-wise manner, given by proteomics. This review summarises the progress of proteomics and its importance for the study of biotechnological processes in filamentous fungi. A major step forward in proteomics was to couple protein separation with high-resolution mass spectrometry, allowing accurate protein quantification. Despite the fact that most fungal proteomic studies have been focused on proteins from mycelial extracts, many proteins are related to processes which are compartmentalised in the fungal cell, e.g. ß-lactam antibiotic production in the microbody. For the study of such processes, a targeted approach is required, e.g. by organelle proteomics. Typical workflows for sample preparation in fungal organelle proteomics are discussed, including homogenisation and sub-cellular fractionation. Finally, examples are presented of fungal organelle proteomic studies, which have enlarged the knowledge on areas of interest to biotechnology, such as protein secretion, energy production or antibiotic biosynthesis
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