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Record number 400872
Title CXCL8 Chemokines in teleost fish: two lineages with distinct expression profiles during early phases of inflammation
Author(s) Aa, L.M. van der; Chadzinska, M.K.; Tijhaar, E.; Boudinot, P.; Verburg-van Kemenade, B.M.L.
Source PLoS One 5 (2010)8. - ISSN 1932-6203 - 13 p.
DOI https://doi.org/10.1371/journal.pone.0012384
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) neutrophil-activating factor - trout oncorhynchus-mykiss - cyprinus-carpio l. - interleukin-8 receptors - molecular evolution - chemotactic factor - escherichia-coli - gene-expression - gro-alpha - tnf-alpha
Abstract Background - During the inflammatory process, chemokine CXCL8 plays a pivotal role in recruitment of human neutrophilic granulocytes. A diversity of sequences similar to CXCL8 was reported in fish, but their evolutionary relationships and functional homology with their human homolog remain unclear. Principal Findings - We screened fish genomes to seek for sequences related to CXCL8. A first lineage was retrieved in all teleosts, while a second CXCL8 lineage was found in zebrafish and carp only. An early inflammatory function for both lineages was indicated by several lines of evidence. The induction of carp CXCL8s, CXCb, and CXC receptor-1 and -2 was analyzed after in vitro stimulation of leukocyte subpopulations and in two in vivo inflammation models. Recombinant proteins of carp CXCL8 proteins were produced and showed significant chemotactic activity for carp leukocytes. Conclusions - While both carp CXCL8s appear to be functional homologs of mammalian CXCL8, their different induction requirements and kinetics evoke a gene-specific sub-functionalization
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