Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 400873
Title Functional analysis of carp interferon-y: Evolutionary conservation of classical phagocyte acativation
Author(s) Arts, J.A.J.; Tijhaar, E.; Chadzinska, M.K.; Savelkoul, H.F.J.; Verburg-van Kemenade, B.M.L.
Source Fish and Shellfish Immunology 29 (2010)5. - ISSN 1050-4648 - p. 793 - 802.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) nitric-oxide synthase - common carp - ifn-gamma - molecular characterization - expression analysis - messenger-rna - tnf-alpha - nf-kb - macrophages - l.
Abstract In teleost fish two IFN-¿ gene sequences were found for which two phylogenetic clusters can be distinguished. Our previous analysis of expression of these in carp led us to hypothesize that a classical IFN-¿ function is associated with the IFN-¿2 cluster. We investigated the evolutionary conservation of the IFN-¿ function, inducing classical activation of phagocytes, thus skewing towards a Th1-like profile of immune activation. Recombinant proteins for the carp IFN-¿ sequences of both clusters were made and we studied their effects on expression of proinflammatory mediators. Carp IFN-¿2, in contrast to carp IFN-¿1, was powerful in inducing a proinflammatory reaction in phagocytes: a classical synergistic response with lipopolysaccharide was observed for the induction of iNOS expression and NO release, for expression of CXCL9-11-like chemokines and the expression of proinflammatory cytokines IL-1ß, TNFa and the IL-12 subunits p35 and p40. In contrast, like in mammals, the CXCL8-like cytokines are LPS but not IFN-¿ sensitive. These results corroborate an evolutionary conserved nature of IFN-¿ function in lower vertebrates including classical activation of phagocytes
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