Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 400936
Title Intestinal T cells of Dicentrarchus labrax (L.): Gene expression and functional studies
Author(s) Picchietti, S.; Guerra, L.; Bertoni, F.; Randelli, E.; Belardinelli, M.C.; Buonocore, F.; Fausto, A.M.; Rombout, J.H.W.M.; Scapigliati, G.; Abelli, L.
Source Fish and Shellfish Immunology 30 (2011)2. - ISSN 1050-4648 - p. 609 - 617.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) trout oncorhynchus-mykiss - lymphoid-tissue galt - cyprinus-carpio l. - rainbow-trout - intraepithelial lymphocytes - mediated cytotoxicity - antigenic determinants - structural-analysis - atlantic salmon - teleost fish
Abstract Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-a, MHCI-a, MHCII-ß, rag-1 and TCR-ß gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.). Real-time PCR findings depicted a main role of the thymus in T-cell development, but also rag-1 and CD8-a transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-ß and CD8-a exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-a(+) cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T-cell-specific gene expressions are first described in the intestine of a bony fish. High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles
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