Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 401175
Title Systemic colonization of potato plants by a soil-borne, GFP-tagged strain of Dickeya sp. Biovar 3
Author(s) Czajkowski, R.L.; Boer, W. de; Velvis, H.; Wolf, J.M. van der
Source Phytopathology 100 (2010)2. - ISSN 0031-949X - p. 134 - 142.
DOI http://dx.doi.org/10.1094/PHYTO-100-2-0134
Department(s) PRI BIOINT Ecological Interactions
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) carotovora subsp atroseptica - erwinia-chrysanthemi - ralstonia-solanacearum - seed potatoes - host-range - pectobacterium - infection - tomato - genes - lenticels
Abstract Colonization of potato plants by soilborne, green fluorescent protein (GFP)-tagged Dickeya sp. IPO2254 was investigated by selective plating, epifluorescence stereo microscopy (ESM), and confocal laser scanning microscopy (CLSM). Replicated experiments were carried out in a greenhouse using plants with an intact root system and plants from which ca. 30% of the lateral roots was removed. One day after soil inoculation, adherence of the pathogen on the roots and the internal colonization of the plants were detected using ESM and CLSM of plant parts embedded in an agar medium. Fifteen days post-soil inoculation, Dickeya sp. was found on average inside 42% of the roots, 13% of the stems, and 13% of the stolons in plants with undamaged roots. At the same time-point, in plants with damaged roots, Dickeya sp. was found inside 50% of the roots, 25% of the stems, and 25% of the stolons. Thirty days postinoculation, some plants showed true blackleg symptoms. In roots, Dickeya sp. was detected in parenchyma cells of the cortex, both inter- and intracellularly. In stems, bacteria were found in xylem vessels and in protoxylem cells. Microscopical observations were confirmed by dilution spread-plating the plant extracts onto agar medium directly after harvest. The implications of infection from soilborne inoculum are discussed.
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