Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 401506
Title Micropropagation of Alstroemeria in liquid medium using slow release of medium components
Author(s) Klerk, G.J.M. de; Brugge, J. ter
Source Propagation of ornamental plants 10 (2010)4. - ISSN 1311-9109 - p. 246 - 252.
Department(s) WUR Plant Breeding
Nursery Stock-Flower Bulbs
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) mass propagation - gelling agent - in-vitro - segments - culture - growth - plants - apple - agar
Abstract Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous atmosphere of the headspace. The medium became rapidly depleted with respect to both organic and inorganic nutrients. Manual addition of extra medium ingredients during the culture enhanced growth. Extra nutrients were also successfully administered via slow-release tools. Two types of slow-release tools were used, Osmocote for inorganic nutrients and newly developed castings for sucrose. Addition of extra 6-benzylaminopurine (BAP) during the culture stimulated the outgrowth of lateral rhizomes. The extra BAP could be included in the slow-release castings for sucrose.
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