Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 401575
Title Micropropagation of dahlia in static liquid medium using slow-release tools of medium ingredients
Author(s) Klerk, G.J.M. de; Brugge, J. ter
Source Scientia Horticulturae 127 (2011)4. - ISSN 0304-4238 - p. 542 - 547.
Department(s) WUR Plant Breeding
Nursery Stock-Flower Bulbs
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) tissue-cultures - suspension-cultures - growth - calcium - formulations - nutrition - segments - necrosis - invitro - apple
Abstract Growth of dahlia shoots in vitro was ca. 4 times faster in liquid medium than on solidified medium. In liquid standard medium (3% sucrose, macroelements according to Driver–Kuniyuki Walnut medium, microelements according to Murashige–Skoog medium, 0.44 µM benzylaminopurine), the major medium ingredients were consumed for 75–80% during the first 6 weeks. Addition of extra ingredients increased growth, demonstrating that the amount of ingredients added at the start of culture was suboptimal. When the extra ingredients were given at the start of the culture, concentrations became too high and therefore inhibitory. When the ingredients were added during the subculture cycle by means of small aliquots of a concentrated solution or by means of slow-release tools, growth was strongly increased. Osmocote gave satisfactory results as a slow-release tool for inorganics. For organic ingredients (sucrose and benzylaminopurine), a novel slow-release tool was developed.
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