Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 401959
Title Molecular cytogenetic mapping of Cucumis sativus and C. melo using highly repetitive DNA sequences
Author(s) Koo, D.H.; Nam, Y.W.; Choi, D.; Bang, J.W.; Jong, J.H.S.G.M. de; Hur, Y.
Source Chromosome Research 18 (2010). - ISSN 0967-3849 - p. 325 - 336.
DOI https://doi.org/10.1007/s10577-010-9116-0
Department(s) Laboratory of Genetics
EPS-4
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) in-situ hybridization - alpha-satellite dna - arabidopsis-thaliana - robertsonian translocations - mitochondrial genome - tandem repeats - evolution - organization - centromere - chromosomes
Abstract Chromosomes often serve as one of the most important molecular aspects of studying the evolution of species. Indeed, most of the crucial mutations that led to differentiation of species during the evolution have occurred at the chromosomal level. Furthermore, the analysis of pachytene chromosomes appears to be an invaluable tool for the study of evolution due to its effectiveness in chromosome identification and precise physical gene mapping. By applying fluorescence in situ hybridization of 45S rDNA and CsCent1 probes to cucumber pachytene chromosomes, here, we demonstrate that cucumber chromosomes 1 and 2 may have evolved from fusions of ancestral karyotype with chromosome number n= 12. This conclusion is further supported by the centromeric sequence similarity between cucumber and melon, which suggests that these sequences evolved from a common ancestor. It may be after or during speciation that these sequences were specifically amplified, after which they diverged and specific sequence variants were homogenized. Additionally, a structural change on the centromeric region of cucumber chromosome 4 was revealed by fiber-FISH using the mitochondrial-related repetitive sequences, BAC-E38 and CsCent1. These showed the former sequences being integrated into the latter in multiple regions. The data presented here are useful resources for comparative genomics and cytogenetics of Cucumis and, in particular, the ongoing genome sequencing project of cucumber
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