Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 402198
Title Effect of prolonged feeding of a sainfoin (Onobrychis viciifolia Scop.) based diet on methanogenic community in the rumen of dairy cows
Author(s) Guglielmelli, A.; Perez, O.; Tiemessen, F.; Domenis, M.; Albanese, S.; Calabrò, S.; Smidt, H.S.; Pellikaan, W.F.
Source In: Listing of Abstracts to be presented at Greenhouse Gases and Animal Agriculture conference, 3-8 October 2010, Banff, Canada. - Banff, Canada : Greenhouse Gases and Animal Agriculture Conference - p. 140 - 141.
Event Banff, Canada : Greenhouse Gases and Animal Agriculture Conference 4th Greenhouse Gases & Animal Agriculture conference, Banff, Canada, 2010-10-03/2010-10-08
Department(s) Animal Nutrition
WIAS
Publication type Abstract in scientific journal or proceedings
Publication year 2010
Abstract An in vivo trial was conducted to investigate the effect of sainfoin tannins on methanogen numbers and rumen fermentation, and to assess adaptive behaviour of rumen microbiota while cows were maintained on sainfoin for an 8-wk period. Three ruminally fistulated dairy cows were placed on a lucerne-based ‘uniformity’ diet for a 2-wk period to allow animals to adapt to a tannin-free legume-based diet. After two weeks, the lucerne was exchanged for sainfoin. During the first 5 d of sainfoin feeding, animals received polyethylene glycol (PEG4000) intraruminally. Thereafter, the animals remained on their sainfoin-based diet for seven more weeks. Samples of rumen fluid were analysed for volatile fatty acids (VFA), ammonia (NH3), the number of protozoa and methanogenic Archaea. There was a significant (P=0.05) decrease in the number of protozoa in the first week after changing to the sainfoin diet. During the subsequent weeks of sainfoin feeding, the number of protozoa showed an increase at days 12, 15 and 37 after PEG treatment, however, their numbers remained numerically lower than during the sainfoin+PEG treatment (6.00 log10/mL). The Archaea followed a similar tendency but animal variation was considerably higher and the decreases were non-significant. Interestingly, this decrease began during PEG administration. Total VFA and NH3 follow a pattern similar to the protozoa numbers during the first week. Total VFA did not differ between the uniformity (124.5 mmol/L) and sainfoin+PEG diet (122.5 mmol/L), but a distinct decrease was observed after PEG treatment was stopped, with greatest effects on day 1 and day 4 (P=0.082). Ammonia showed a numerical decrease when animals changed from uniformity diet (138 mg/L) to the sainfoin+PEG diet (85.4 mg/L; P=0.267), followed by a further decrease during the first five days after stopping PEG treatment (54.4 mg/L; P=0.088). Results suggest that PEG may not have been fully successful in completely blocking the effect of tannins, which is reflected in the numerical decline in protozoa and Archaea during PEG administration. Sainfoin tannins resulted in a partial inhibition of protozoa and methanogens. The initial decrease and successive increase in total VFA suggests that fermentation activity is not negatively affected by sainfoin. The consistent lower ruminal NH3 with sainfoin compared to lucerne suggests a protective effect of tannins on dietary protein. The tendencies of the different parameters suggest that the microbial population (protozoa, Archaea, other bacteria) respond in different ways to the sainfoin diet over time, suggesting adaptation to the dietary conditions
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