Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 403076
Title Evaluation of three commercial enzyme-linked imuunosorbent assays for the detection of antibodies against Salmonella spp. in meat juice from finishing pigs in Spain
Author(s) Vico, J.P.; Engel, B.; Buist, W.G.; Mainar-Jaime, R.C.
Source Zoonoses and Public Health 57 (2010)Suppl. 1. - ISSN 1863-1959 - p. 107 - 114.
Department(s) Biometris (WU MAT)
Biometris (PPO/PRI)
CVI - Division Virology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) diagnostic-tests - enterica prevalence - control program - slaughter pigs - swine - elisa - surveillance - culture - herds - infection
Abstract The control of animal salmonellosis is considered as a major objective in Europe and indirect ELISAs will be important tools for the implementation of control programs for this infection in pigs. We analyse the results yielded by three commercial ELISAs (Herdcheck Swine Salmonella, SALMOTYPE Pig Screen, and PrioCHECK Salmonella) on meat juice samples from a population of slaughter pigs of Aragon, NW Spain, to assess their efficacy using traditional and latent-class approaches. Overall, the Herdcheck Swine Salmonella detected more Salmonella-infected pigs than the other two tests, but its relative sensitivity was low (65.9%). A similar result was observed when only serotypes detectable by this test were considered (69.1%). When a Bayesian approach was used the Herdcheck Swine Salmonella showed also the highest overall accuracy (sensitivity = 88% and specificity = 74%). Our results suggest that a relatively small proportion of the observed prevalence in herds would be explained by using these ELISAs. Also, this study points out that when different ELISA tests are used within the same herd, results may differ substantially. Thus, caution is advised if it is decided to use these assays for herd health classification in Spanish Salmonella control programs
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