Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 403320
Title Insect Cell Culture
Author(s) Oers, M.M. van; Lynn, D.E.
Source In: Encyclopedia of Life Sciences John Wiley and Sons - ISBN 9780470066515
DOI http://dx.doi.org/10.1002/9780470015902.a0002574.pub2
Department(s) Laboratory of Virology
PE&RC
Publication type Chapter in scientific book
Publication year 2010
Abstract Insect cell cultures are widely used in studies on insect cell physiology, developmental biology and microbial pathology. In particular, insect cell culture is an indispensable tool for the study of insect viruses. The first continuously growing insect cell cultures were established from lepidopteran insects around 1960. Since then, more than 600 insect cell lines have been described from over 100 insect species, the majority from lepidopteran (moths) and dipteran (flies and mosquitoes) insects. Among these, the Drosophila S2, the Spodoptera frugiperda Sf9 and Trichoplusia ni High Five cell lines are used most widely. The latter two are susceptible to the baculovirus Autographa californica multiple capsid nucleopolyhedrovirus and are used for the expression of foreign genes, for example, for the production of subunit vaccines, to make proteins for functional analyses or crystallography and to produce gene delivery vectors for mammalian cells.
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